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Two photon microscopy vs confocal microscopy

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Larger amounts of glomerular albumin filtration have been reported. One study using tritium-labeled albumin demonstrated a sieving coefficient of 0.074, and another study using Alexa-labeled albumin observed by confocal microscopy resulted in a sieving coefficient of 0.0341, which is 50 to 100 times higher values than previous studies. However, there were several technical limitations.





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